tufA [2019-06-07 17:39:56]

elongation factor Tu

locus: BSU01130

pI: 4.72

mw: 43.43 kDa

protein length: 396 aa

gene length: 1191 bp

function: translation

product: elongation factor Tu

essential: yes

ec: 3.6.5.3

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 132,882 → 134,072

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Protein family

• EF-Tu/EF-1A subfamily (according to Swiss-Prot)

Modification

• phosphorylated on Thr-384 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|PrpC] [Pubmed|19246764], [Pubmed|17726680]
• Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species [Pubmed|22938038]
• phosphorylated on several Arg residues [Pubmed|24263382]
• phosphorylated during [SW|sporulation] on Thr-63 by [protein|E86A96B832351FF513DD9853EAD8998CC44C9951|YabT], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|PrpC] [Pubmed|26056311]
• phosphorylated on Tyr-270 [Pubmed|26381121]

Effectors of protein activity

• phosphorylation by YabT results in inhibtion of GTP hydrolysis; as a result, EF-Tu remains stably bound
• to the [SW|ribosome], stalling protein [SW|translation] [Pubmed|26056311]

Structure

• [PDB|3U6B] (from ''E. coli '', 77% identity) [Pubmed|21999529]

[SW|Localization]

• EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] [Pubmed|20133608]
• membrane [Pubmed|18763711]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]-[gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL]-[gene|8049CACE85B2627A08CB4D8A8587D52E2DB24C7F|rpsG]-[gene|297844645CCCF40CB40E84339997F6610883DF4E|fusA]-[gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|tufA]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the mRNA is processed between [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB] and [gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

Expression vectors

• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP839, available in [SW|Stülke] lab
• for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP847, available in [SW|Stülke] lab

References

• 22938038,19246764,20133608,25707802,19246764,17726680,18763711,24263382,15378759,25676310,26056311,26381121,11918677,28516784,30394869